Meeting Time:
Section 01- 2:30-5:20 pm W
Section 02- 9:00-11:50 am R
Section 03- 2:10-5:00 pm R
Meeting Place: Ulmer 304
Instructor: Dr. Jackie Whitling (section 02)
Professor of Chemistry
Office: 304B Ulmer Hall or Honors 23
Phone: 570-484-3045
e-mail: jwhitlin@lhup.edu
URL: http://www.lhup.edu/jwhitlin/home.htm
Dr. Whitling's Office Hours:
Monday: 10:00 - 11:30 am Honors 23; 1:30 - 2:30 Ulmer 304B
Wednesday: 3:00 - 4:00 pm Honors 23
Friday: 10:30 am - 12:00 pm Ulmer 304B
Instructor: Dr. Stephen Coval (sections
01, 03)
Office: 404B Ulmer Hall
Phone: 570-484-2061
email: scoval@lhup.edu
Dr. Coval's Office Hours:
Tuesday & Thursday: 10:00 - 11:30
Wednesday: 11:00 - 12:00
(Office Hours also available by appointment)
Prep time for laboratory: Dr. Coval and I will not be available for assistance one to two hours before each lab as there are time sensitive reagents that must prepared during this time. Thanks for your understanding.
Required Materials:
Note: Some experiments are in the lab pack distributed at the beginning of the semester, some may be handouts given by the instructor prior to the experiment, while others are found in the laboratory manual. The basic principles and theories for the majority of experiments are included in the laboratory manual. Therefore, experiment numbers do not correspond to those in the laboratory manual. The page numbers of assigned readings (from lab manual) for each experiment is listed along with the title of the experiment and if the experiment is in the lab pack, a handout, or an experiment from the laboratory manual (page numbers are listed). You are expected to read all materials before your laboratory session and complete any pre-lab material assigned by the instructor. Any questions about a particular experiment, feel free to contact the instructor.
Date: Experiment #/Title Required Readings Format of Expt. Jan 25, 26 Lab basics and Check-in Jan 25 MLK, Jr. Celebration- Classes follow
compressed schedule. Wed. lab meets 3:15 - 5:45
pm Feb 1, 2 Ex 1- Using pipettors &
Use of PC's to Prepare Lab Reports (MS
Excel & Word, ChemDraw) pages 1-28 Lab pack; collecting notebook copy
pages Feb 8, 9 Ex 2- Spectrophotometry pages 63-73 Lab pack Feb 15, 16 Ex 3- Electrophoresis
(SDS-PAGE)* pages 239-250 pp. 263-268** Feb 22, 23 Ex 4- Week 1: Preparation of Standard
Curves (BSA and PNP) pages 67-73 Lab pack & pg 76-77 Feb 29, Mar 1 Ex 4- Week 2: Partial Purification of
Wheat Germ Acid Phosphatase (Gel filtration) pages 181-188 Lab pack Mar 7, 8 Ex 4- Week 3 Data Analysis Lab pack Mar 14, 15 NO LABS- Spring Break Mar 21, 22 Ex 5- Week 1: Enzyme Kinetics of
Tyrosinase including Inhibitor Studies pages 211-220 Mar 28, 29 Ex 5- Week 2: continued pages 211-220 pp. 221-229 & lab pack Apr 4, 5 Ex 6- Week 1: Western Blot of Serum
Proteins* pages 277-280 pp. 281-283** Apr 11, 12 Ex 6- Week 2: continued pages 277-280 pp. 281-283** Apr 18, 19 Biochemical modeling lab & clean
up/checkout Web MO, Protopedia, BioOra Handout Apr 25, 26 COS alternate
assignment Labs will not be held this
week. May 2, 3 Lab Assessment, All Reports
Due Lab practical details TBA
(Beers Law, Determination of vitamin C in every day
products)
*- Experiment may need to be completed the following day or results may need to be recorded. Instructor will notify you with further instructions.
**- Instructions will be given on how to assemble apparatus in the lab pack.
Expectations: Before You Enter
Lab
Please be sure to read the experiment you will be performing
including any assigned readings on theory or technique. If
pre-laboratory questions have been assigned, please have these
completed as well. You do not have to include a detailed procedure
or safety/data tables in your notebook. However, you should have
an outline of the procedure written on loose-leaf paper that
allows you to better utilize your time in the laboratory and complete
the experiment in the allotted time unit. Since you will be working
with a partner, it would be wise to decide how to divide up the work
evenly prior to the experiment. You should also list what chemicals
and equipment you will be using and the best method for dispensing
these chemicals, if not specified in the experiment (i.e. automatic
pipet, pipet pump, graduated cylinder, etc.) on your looseleaf pages.
In your notebook, you may fill in the date, title, and purpose of the
experiment to be performed. No other information should be in the
notebook.
Recording and Reporting Data
Please refer to the Department of Chemistry laboratory notebook
guidelines found in the Lab Pack. The laboratory notebook may be used
when instructor assigns technique points, i.e. carbon copies may
be collected upon request of the instructor. Since you will be
working with a partner, it is best that each person records their own
observations and that both of you have all data and results in your
notebook before you leave the lab. This will save time when writing
your lab report later. Late lab reports will not be accepted due to
not obtaining the information from your partner.
Laboratory Reports
All lab reports writing style must follow a peer-reviewed scientific
journal article format. Please refer to the two journal articles and
sample report in the lab pack for examples. When writing your lab
reports, keep in mind that I will be looking for quality rather than
quantity. I am more interested in the number of good thoughts that
you express rather than the number of pages that you turn in.
Demonstrate to me that you are thinking about your experiments,
rather than rewriting things that I have already told you. Each lab
report is to be typed and double spaced. All graphs and
figures should be generated with a computer. The following
sections are typical of a journal article. The instructor will assign
which sections are to be completed for each experiment.
Majority of report should be written in full paragraph form, past
tense, and third person. For example, do not use I, we, you etc.
Exceptions to when you can use first person is when stating your
goals/purpose in the introduction and in the discussion when you are
specifically making reference to your results i.e. "the data we
obtained indicates that the sample contained ...." or "our results
imply that ... ". First person should NOT be used at any time in the
methods or results. Remember, scientific writing is different from
literary writing. The style is more factual and less personal. Proper
scientific formatting should also be used i.e. correct use of units,
significant figures, captions for figures and graphs.
1. Title Page (This is one exception to the journal article style and will always appear as a separate page.)
a. Title of experimentb. Name
c. Course name
d. Due date of experiment
2. Abstract (150 - 250 words)
3. Introduction
a. Background (i.e. theory on techniques used; biochemical significance of analyte; background on the reaction assay; other relevant material)b. Objective (purpose and importance of experiment)
4. Materials and Methods (Experimental Procedures)
a. Materials (list of pertinent reagents including concentration and pH; name, model and manufacturer of major equipment)b. Methods (summary of procedure in your own words; should be written in paragraph form, past tense, third person)
5. Results*
This section will contain all figures, tables, graphs, sample calculations and experimental results.
6. Discussion*
a. Overall summary statement about the experiment and results obtainedb. Critical evaluation of data presented in results including conclusions based on results
c. Significance of your results (i.e. comparison to the literature)
d. Future directions (i.e. additional experiments/trials)
7. Acknowledgements (always acknowledge contribution of partner)
8. References (citation numbers must be included in document; format of references should follow that of a scientific journal article**; you should cite at least two references from scholarly peer-reviewed journals)
* Specific information for each experiment will be given in the laboratory or posted on the website. Mistakes/errors that occurred should NOT be included in your discussion but should be documented in your notebook.
** Two example journal articles from peer-reviewed biochemical journals are found in the lab pack. There is also a sample lab by a former student.
Deadlines: Typically, laboratory reports will be due one week after the date the experiment was completed unless instructed otherwise. Points will be deducted for late lab reports. (minus 10% of total points for each week late)
Grading Policy: The laboratory grade is not a separate grade from the course. The total points from the laboratory have been incorporated into the total points for the course.
Lab reports*: Experiments 2, 3, 6, modeling lab, COS
assignment 15 points ea 75 pts Experiments 4, 5 25 points ea 50 pts Technique: 20 pts Lab Assessment: 20 pts Writing Emphasis: 10 pts Total: 175 pts
*A portion of each laboratory score will be allotted to writing
emphasis. A maximum of 10 points will also be allotted to the total
point score for the laboratory. Articles from two biochemical
journals are provided in the lab pack. Critique will be provided by
the instructor on each report submitted. A grading rubric for writing
assessment is also provided in the lab pack. Assistance in improving
writing skills is available by the instructor and the LHUP Writing
Center located on the 2nd floor of the Stevenson Library, 484-3598.
Students will also have the opportunity to rewrite one report
designated by the instructor.
Attendance: ATTENDANCE is MANDANTORY!! This includes arriving to the laboratory on time! Important information regarding the experiment is typically given during the first 15-20 minutes. Therefore, it is not wise to be late. You must come to the section that you are assigned. There is limited space in lab and it is not fair to other students to allow a student to make up a missed lab during another section. Please notify me if you know in advance that you will miss a laboratory or as soon as possible after you miss a lab. It is your responsibility to notify me. A valid University approved excuse must be provided for all missed laboratories. All unexcused absences will result in a zero for that experiment!
Academic Dishonesty Policy: Although you are working in pairs, and I want you to discuss your experiments with each other, I expect you to write your reports individually. Thus, you should be the only author of your report. Assistance from your lab partners, the writing center, etc. can be acknowledged in the acknowledgments section. Submission of a plagiarized report will receive a zero. Refer to the University Student Handbook on academic dishonesty policies.
Students with Disabilities Reasonable Accommodations: Students with disabilities are encouraged to discuss requests for reasonable accommodations with the professor at the beginning of the semester. In order for accommodations to be provided, your disability must be verified and documented. Please contact Dr. Reynol Junco (484-2926, Russell 104, rjunco@lhup.edu) and the secretary for Disability Services for Students, Jean Mason (484-2665, Russell 112, jmason@lhup.edu) for assistance.
Electronic Device Policy: Out of courtesy, students are not permitted to use cell phones, headsets, or other electronic devices at any time during the class unless authorized by the instructor or disability services. Please turn off all devices and remove headsets prior to the start of class.
Incement Weather Schedule: Please refer to the University website for inclement weather information and compressed schedule. http://www.lhup.edu/weather.htm Note: Section 02 lab on Thursday would begin at 10:45 am in the event of a delay.
Emergency: In the event of an emergency, please dial 9-911.