Cell and Molecular Biology BIOL330 Fall
2009
Text: Unraveling DNA, Molecular
Biology for the Laboratory. Winfrey,
Rott, and Wortman,
Prentice-Hall, Inc. 1997
Bring lab book, notebook, MCJ,
calculator, gloves, and sharpie to each lab class.
LABORATORY
SCHEDULE (Room U309)
Date Lab Topic
Sept.
2-3 4 !
Restriction Digestion and Agarose Gel Electrophoresis of DNA
(Sept 2 lab times due to Convocation:
3:15-5:45pm)
9-10 5 Isolation of Chromosomal DNA from Vibrio
fischeri
16-17
5 Continued
23-24 7 W Spectrophotometric Analysis of DNA (from Lab 5)
8 !
Digestion of Vibrio fischeri
Genomic DNA and Plasmid Vector
30-1 9 Quantification of Genomic DNA (from
Lab 8)
10 Ligation of Restriction Fragments of Vibrio fischeri DNA
to a Plasmid Vector
Oct. 7-8
10 ! Ligation
of Restriction Fragments of Vibrio fischeri DNA to a Plasmid Vector
Overview of Cloning (Lecture
Text, Chapter 10)
14-15 12 + Transformation of Competent Escherichia
coli DH5α
with Recombinant Plasmids (from
Lab 10)
Screening
the Vibrio fischeri
Genomic Library for Light Producing Clones
21-22 23 Overview of PCR (Lecture Text, Chapter
10)
Amplification
of luxA by PCR
28-29 23 Gel Analysis of PCR Amplification
16 Southern Blotting
Nov. 4-5 23 ! Hybridization (Lecture Text, Chapter 10)
11-12
PCR Experiments: experimental design, master mix math
18-19
PCR setup and run
25-26 -- THANKSGIVING -- NO LABS!!!!!!
2-3 ! PCR gel
Dec.
9-10
Full Lab Review for Final Exam. Final
Math Evaluations.
Note: This schedule is subject to
modification. Laboratory exercises must be
read before class. Attendance is
mandatory. Bring lab book, notebook,
calculator, MCJ, gloves, and sharpie to class. + = Lab continued on your own the next
day(s). ! = Performance evaluation. W = Written report due the next week. Pop quizzes may be given on calculations,
procedure theory, and current lab procedure.
Attire: For lab, closed-toe shoes, gloves,
and safety glasses are needed.
Long pants are suggested.
Radios/walkman are
not permitted during class. NO eating,
drinking, or tobacco-products in the lab.
Cell and Molecular Biology BIOL330 Fall 2009
Instructor: Dr.
Office:
Ulmer Hall 307 Web
Site: www.lhup.edu/tnuttall/
Phone: 484-2058 Office Hours: M11- 12am, 1-3pm
W,F 11 - 12am
Cell Phone use is FORBIDDEN during lecture and lab. All electronic devices should be stored and not in site, not used, or even touched.
Class and lab time is for learning, not chatting. Use of electronics (phones, music, etc.) during class is a safety hazard, and shows a lack of concentration on the task at hand that will be mentioned in letters of reference.
Attendance Policy: Laboratory attendance is mandatory. Missing more than one lab may result in dismissal from class.
Special
Help: If you have a learning disability, I will
work with you and Student Support Services to accommodate all the adaptations
necessary. I want you to succeed! Please bring your disability documentation to
me as soon as possible. Remember, in order for accommodations to be
provided, your disability must be verified by the Office for Disability Services,
104 Russell Hall, 484-2665.
Email: All email correspondences will be made to
campus email addresses only. Please
be sure to activate your LHU account and check it.
Exams
& Quizzes:
All exams and quizzes must be taken on schedule. No make-up exams or quizzes will be given
unless prior arrangements with me are made as far in advance of the exam as
possible. Only official LHU excuses are
acceptable.
Cheating: Please don't!
If you are caught cheating (this includes plagiarism) you will receive a
zero on that assignment or exam. If you
are caught a second time, you will immediately fail the class. Read the Academic Honesty policy in the
student handbook. Cheating is an insult
to everyone who studies hard and works honestly.
Laboratory
conduct: Do not play around with equipment or
supplies—they are expensive and sometimes dangerous! Stealing or
intentionally misusing/breaking supplies or equipment will result in suspension
from the class. Additionally,
intentional classroom disruptions will not be tolerated—please be courteous to
your fellow students.
Grading: Lecture
Exam I 100pts
Exam II 100pts
Exam III 100pts
Quizzes (6) 60pts
Laboratory Lab Performance 40pts
(may be higher with writing assignments)
Lab Performance Final 100pts
TOTAL 500pts*
(The total may be different due to extra quizzes and writing assignments in lab)
Performance Points: Up to 20 performance points can be added to your total points.
Grades:
A = >90% A - = 88-89.9 B+ = 86-87.9 B = 80-85.9 B
- = 78-79.9
C+ = 76-77.9 C = 70-75.9 C - = 68-69.9 D+
= 66-67.9 D = 60-65.9 E = <60
Laboratory
Skills that you should become proficient in (I will assess you in the final
exam):
1.
Preparing
complex working solutions from stock solutions.
Understand unit conversions.
2.
Write
a procedure in journal format.
3.
Determine
the amount of reagent needed to achieve a given final concentration.
4.
Using
a micropipette correctly. Understand
unit conversions.
5.
Describe
the definitions of common laboratory terms (pulsing, dilutions, stock,
blotting, PCR, etc.).
6.
Know
why a chemical or condition is used in a procedure.
7. Interpret or predict results in the form of absorption spectra, gel picture, etc.
8.
Set
up reactions such as ligation, restriction digests,
PCR reactions, etc.
9.
Understand
how to use various types of enzymes correctly.
10. Use
various types of laboratory equipment correctly.
11. Know
the safety rules for lab work, including the dangers of common chemicals.