BIOL345 Advanced DNA Methods Spring 2013
Text: Butler, JM. 2005. Forensic DNA Typing. 2nd Edition. Elsevier Academic Press, Oxford.
Instructor: Dr. Ted Nuttall E-mail: email@example.com
Office: Ulmer Hall 307 Web Site: www.lhup.edu/tnuttall/index.htm
Phone: 484-2058 Office Hours: M 10- 11; T, 9-11, 2-3; F 9-10
First half of the semester: DNA Sequencing (using AFDIL protocols) Book chapter 10-11, 12-14
Isolate DNA, PCR amplify mtDNA, sequence analysis on 310 Genetic Analyzer
Second half of the semester: Genotyping (using Profiler Plus) Book chapters 1-7, 12-15
Isolate DNA, PCR amplify multiple loci, fragment analysis on 310 Genetic Analyzer
Bring to Class: Text book, Notebook for general note-taking, Dedicated lab notebook (spiral bound, leave in lab), Sharpie (extra fine is best), Calculator, Gloves.
Reasonable Accommodations: Students with disabilities are encouraged to discuss requests for reasonable accommodations with me at the beginning of the semester. In order for accommodations to be provided, your disability must be verified by Heather Fowler, Interim Director of Disability Services, 104 Russell Hall, 570-484-2926, firstname.lastname@example.org.
Email: All email correspondences will be made to campus email addresses only. Please be sure to use your LHU account and check it.
Exams & Quizzes: All exams must be taken on schedule. No make-up exams will be given unless prior arrangements with me are made as far in advance of the exam as possible. Only official LHU excuses are acceptable. All quizzes will begin a few minutes after the beginning of class, and may be unannounced. Some may include finding information in your lab manual. Be on time! No make-up quizzes will be given.
Cheating: Please don't! If you are caught cheating (this includes plagiarism) you will receive a zero on that assignment or exam. If you are caught a second time, you will immediately fail the class. Read the Academic Honesty policy in the student handbook. Cheating is an insult to everyone who studies hard and works honestly.
Laboratory conduct: Do not play around with equipment or supplies—they are expensive and sometimes dangerous! Stealing or intentionally misusing/breaking supplies or equipment will result in suspension from the class. Additionally, intentional classroom disruptions will not be tolerated—please be courteous to your fellow students.
Grading: (The actual total may be different due to extra quizzes in lab.)
Exam 1 100pts last week in February
Exam 2 100pts finals week: 2-3:50pm, Tuesday, May 14
Lab notebook 20pts
Exams may contain hands-on tasks, such as genotype/sequence interpretation, instrument use, and problem solving. Exams will be open book and open note. Quizzes will be closed everything.