BIOL345                                                Advanced DNA Methods                                                              Spring 2013

 

Text:  Butler, JM. 2005. Forensic DNA Typing. 2nd Edition. Elsevier Academic Press, Oxford.

 

Instructor:           Dr. Ted Nuttall                                   E-mail:                  tnuttall@lhup.edu

Office:                  Ulmer Hall 307                                   Web Site:            www.lhup.edu/tnuttall/index.htm

Phone:                 484-2058                                              Office Hours:     M 10- 11; T, 9-11, 2-3; F 9-10

 

First half of the semester:   DNA Sequencing (using AFDIL protocols)      Book chapter 10-11, 12-14

                Isolate DNA, PCR amplify mtDNA, sequence analysis on 310 Genetic Analyzer

 

Second half of the semester:     Genotyping (using Profiler Plus)         Book chapters 1-7, 12-15

                Isolate DNA, PCR amplify multiple loci, fragment analysis on 310 Genetic Analyzer

 

Bring to Class:  Text book, Notebook for general note-taking, Dedicated lab notebook (spiral bound,      leave in lab), Sharpie (extra fine is best), Calculator, Gloves.

Reasonable Accommodations:  Students with disabilities are encouraged to discuss requests for              reasonable accommodations with me at the beginning of the semester. In order for              accommodations to be provided, your disability must be verified by Heather Fowler, Interim    Director of Disability Services, 104 Russell Hall, 570-484-2926, hfowler@lhup.edu.

Email:  All email correspondences will be made to campus email addresses only.  Please be sure to use                 your LHU account and check it.

Exams & Quizzes:  All exams must be taken on schedule.   No make-up exams will be given unless prior                 arrangements with me are made as far in advance of the exam as possible.  Only official LHU      excuses are acceptable.  All quizzes will begin a few minutes after the beginning of class, and              may be unannounced.  Some may include finding information in your lab manual.  Be on time!        No make-up quizzes will be given. 

Cheating:  Please don't!  If you are caught cheating (this includes plagiarism) you will receive a zero on   that assignment or exam.  If you are caught a second time, you will immediately fail the class.           Read the Academic Honesty policy in the student handbook.  Cheating is an insult to everyone     who studies hard and works honestly.

Laboratory conduct:  Do not play around with equipment or supplies—they are expensive and sometimes dangerous! Stealing or intentionally misusing/breaking supplies or equipment will    result in suspension from the class.  Additionally, intentional classroom disruptions will not be       tolerated—please be courteous to your fellow students.

Grading:   (The actual total may be different due to extra quizzes in lab.)

                                Exam 1                                  100pts                   last week in February

                                Exam 2                                  100pts                   finals week:  2-3:50pm, Tuesday, May 14

                                Lab notebook                      20pts

                                Quizzes                                                  40pts

                                TOTAL                                   260pts

Exams may contain hands-on tasks, such as genotype/sequence interpretation, instrument use, and problem solving.  Exams will be open book and open note.  Quizzes will be closed everything.