Pipetting Table problems

1. Set up a pipetting table for 3 PCR tubes so that each contains 15ul master mix, primers at 0.16uM (from 100uM), and 2, 4, or 8ng of DNA (from 280ug/ml). Final PCR volume will be 50ul.

2. Set up a pipetting table for 4 PCR reactions at 40ul each so that each contains 10ul master mix, primers at 0.2uM (from 10uM), 1ng of DNA (from 65ug/ml), and 2, 4, 6, or 8mM MgCl2 (from 25mM).

3. Set up a PCR table to test whether Potassium has an effect on PCR. Test 0, 1, 5, 10mM KCl (from a 1M KCl solution) along with 25ul master mix, 5ng of DNA (150ng/ul), and 0.12uM each primer (10uM) in a total PCR volume of 50ul.

4. How concentrated are the reagents in the master mix for the above problems?

1. 2. 3.

5. What reagents do you expect to be in the master mix for the above problems?

1.

2.

3.